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| Registros recuperados: 10 | |
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| Simon-colin, Christelle; Alain, Karine; Colin, Sebastien; Cozien, Joelle; Costa, B; Guezennec, Jean; Raguenes, Gerard. |
| Aims: The aim of the present study was to describe an aerobic, mesophilic and heterotrophic bacterium, designated RA26, able to produce a medium-chain-length polyhydroxyalkanoate (PHA). It was isolated from a French Polynesian bacterial mat located in the atoll of Rangiroa. Methods and Results: This micro-organism, on the basis of the phenotypical features and genotypic investigations can be clearly assigned to the Pseudomonas genus and the name of Pseudomonas guezennei is proposed. Optimal growth occurs between 33 and 37 degrees C, at a pH between 6 4 and 7 1 and at ionic strength of 15 g 1(-1) of sea salts. The G+C content of DNA is 63.2%. Under laboratory conditions, this bacterium produced a novel, medium-chain-length PHA, mainly composed of... |
| Tipo: Text |
Palavras-chave: Pseudomonas; Polyhydroxyalkanaote; Microbial mat; Kopara. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-3858.pdf |
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| Simon-colin, Christelle; Raguenes, Gerard; Crassous, Philippe; Moppert, X; Guezennec, Jean. |
| Pseudomonasguezennei biovar. tikehau was isolated from a microbial mat on the atoll of Tikehau in French Polynesia, and is able to synthesize medium chain length poly-beta-hydroxyalkanaote copolymers when grown on coprah oil. A two-step cultivation process was used and the biosynthesis of PHAs was followed along 52 h by regular culture sampling. The polyester was purified from freeze-dried cells and analysed by nuclear magnetic resonance (NMR), Fourier transform infra red (FTIR), and gas chromatography mass spectrometries. The copolyester produced by P. guezennei biovar. tikehau from coprah oil mainly consisted of saturated monomers, i.e. 3-hydroxyoctanoate (3HO) and 3-hydroxydecanoate (3HD), and the monomeric composition of the polyester did not change... |
| Tipo: Text |
Palavras-chave: Polyhydroxyalkanaote; PHAs; Pseudomonas; "kopara"; Microbial mat. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-6119.pdf |
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| Leroy, Celine; Compere, Chantal; Delbarre Ladrat, Christine; Raguenes, Gerard; Ghillebaert, François; Combes, Didier. |
| Une nouvelle bactérie marine du genre Pseudoalteromonas sp., D41, formant rapidement des biofilms sur tout type de matériau (verre, acier inoxydable, Téflon) a été isolée en Baie de Brest. L’objectif de ce présent travail est de caractériser et décrire cette bactérie capable de produire des exopolymères constitués en majorité de protéines. Le rôle des protéines sécrétées, dans les mécanismes d’adhésion, est discuté. |
| Tipo: Text |
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| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/00088/19950/17617.pdf |
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| Simon-colin, Christelle; Raguenes, Gerard; Costa, Bernard; Guezennec, Jean. |
| The biosynthesis of poly-(3-hydroxyalkanoates) (PHAs) by Pseudomonas guezennei using glucose and/or fatty acids with chain-length from 3 to 18 carbon atoms as carbon sources was investigated. Glucose, acetate, pyruvate, propionate, valerate, hexanoate, heptanoate, octanoate, decanoate, and oleic acid were supplemented in the mineral medium as the sole carbon source or as a mixture for PHAs accumulation. Chemical composition of the resulting PHAs was analysed by coupled gas chromatography mass spectroscopy (GCMS), Fourier Transform Infrared Spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR). PHAs contained up to 10 different types of 3-hydroxyalkanoic acid units, including saturated 3-hydroxyacids from C4 to C14, and unsaturated monomers... |
| Tipo: Text |
Palavras-chave: Carbon substrates; Pseudomonas guezennei; Polymer characterization; Polyhydroxyalkanoates. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-6115.pdf |
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| Alain, Karine; Pignet, Patricia; Zbinden, M; Quillevere, M; Duchiron, F; Donval, Jean-pierre; Lesongeur, Francoise; Raguenes, Gerard; Crassous, Philippe; Querellou, Joel; Cambon-bonavita, Marie-anne. |
| A novel thermophilic, anaerobic, strictly chemoorganoheterotrophic bacterium, designated as AM1114(T), was isolated from a deep-sea hydrothermal vent sample from the East-Pacific Rise (EPR 13degrees N). The cells were long (3-10 mum) rods, motile with peritrichous flagella, and exhibited a Gram-negative cell wall ultrastructure. In the late stationary phase of growth, cells formed an ovoid, refractile, terminal endospore. They grew at 45-65 degreesC inclusive (optimum 55-60 degreesC; doubling time approx. 45 min), at pH 4.5-8.0 inclusive (optimum pH 7.5-8.0) and at sea salt concentrations of 20-60 g l(-1) inclusive (optimum 25-30 g l(-1)). Strain AM1114(T) was an obligately heterotrophic bacterium able to ferment a mixture of 20 amino acids, complex... |
| Tipo: Text |
Palavras-chave: Caminicella sporogenes; Clostridiales; Thermophile; Deep sea hydrothermal vent. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-528.pdf |
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| Moppert, Xavier; Le Costaouec, Tinaig; Raguenes, Gerard; Courtois, Anthony; Simon-colin, Christelle; Crassous, Philippe; Costa, Bernard; Guezennec, Jean. |
| A bacterium isolated from microbial mats located on a polynesian atoll produced a high molecular weight (3,000 kDa) and highly sulphated exopolysaccharide. Previous studies showed that the chemical structure of this EPS consisted of neutral sugars, uronic acids, and high proportions of acetate and sulphate groups. The copper- and iron-binding ability of the purified pre-treated native EPS was investigated. Results showed that this EPS had a very high affinity for both copper (9.84 mmol g(-1) EPS) and ferrous iron (6.9 mmol g(-1) EPS). Amazingly, this EPS did not show any affinity for either ferric ions or selenium salts. This finding is one of the first steps in assessing the biotechnological potential of this polysaccharide. |
| Tipo: Text |
Palavras-chave: Sulphated exopolysaccharide; Microbial mats; Iron; Copper; Binding capacity. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6397.pdf |
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| Guezennec, Jean; Moppert, Xavier; Raguenes, Gerard; Richert, Laurent; Costa, Bernard; Simon-colin, Christelle. |
| It is well known that microorganisms well-adapted to survival in extreme ecosystems could be considered as new sources of biomolecules that have biotechnological importance. On French Polynesian atolls, microbial mats are developing in water ponds exposed to fluctuations in physical and chemical parameters. In these microbial mats, which are called "kopara" by the inhabitants, bacteria coexist with cyanobacteria, and a synergistic relationship may exist between these two types of living microorganisms. A large number of cyanobacteria and bacteria have been isolated from different mats. Under laboratory conditions, these microorganisms were shown to produce various exopolymers, including exopolysaccharides and poly-beta-hydroxyalkanoates, along with... |
| Tipo: Text |
Palavras-chave: Microbial mats; Kopara; Biopolymers; Exopolysaccharides; Poly-beta-hydroxyalkanoates. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00029/14058/11423.pdf |
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| Registros recuperados: 10 | |
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